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| Effects of Low Temperature Pretreatment on Anther Culture and Physiological Index in Pepper |
| DING Zijian1 ，ZHANG Junhua1 ，WU Lili1 ，TANG Xiaoling1 ，JIANG Qingqing1 ，CHENG Weitao1 ，YANG Bozhi1*，ZHOU Shudong2 |
| 1 College of Horticulture，Hunan Agricultural University，Changsha 410128，Hunan，China；2 Hunan Vegetable Research Institute，Changsha 410125，Hunan，China |
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Abstract In order to further improve the anther culture efficiency of pepper，taking pod pepper XR1 as the experimental material，the eff ects of pretreatment of fl ower buds at normal temperature（25 ℃，CK）and low-temperature（0、4、8 ℃）on the efficiency of XR1 anther culture and changes in physiological indicators were studied．The results showed that compared with normal temperature，after low-temperature pretreatment of flower buds，the anther browning rate was significantly reduced，and the induction rate of embryoids was significantly increased．In addition to the significantly reduced MDA content in anthers，the contents of stress-resistant substances（soluble proteins，soluble sugars，Pro）and the activities of antioxidant enzymes （POD，PPO）were signifi cantly increased．The anther browning rate was the lowest under 0 ℃ treatment，the induction rate of cotyledon-shaped embryoids was the best under 4 ℃ treatment，and the induction rate of non-cotyledon-shaped embryoids was the best under 8 ℃ treatment．Among the anthers，except for the highest MDA content under 0 ℃ treatment，the other physiological indicators reached the highest values under 4 ℃ treatment．Correlation analysis showed that there was a negative correlation between the anther browning rate and the induction rate of embryoids（cotyledon-shaped and non-cotyledon-shaped），and there was an extremely signifi cant negative correlation with the MDA content and the strongest correlation，with a correlation coeffi cient of -0.967．The induction rates of both cotyledon-shaped and non-cotyledon-shaped embryoids were extremely significantly positively correlated with the soluble sugar content in the resistance index and had the strongest correlation，with correlation coefficients of 0.837 and 0.784，respectively．Only cotyledon-shapedembryoids could induce tissue culture seedlings during anther culture，and the pretreatment of flower buds at 4 ℃ had the best eff ect．The research results can provide a theoretical basis for in-depth analysis of the benefits of low-temperature pretreatment in improving the efficiency of pepper anther culture．
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Received: 28 December 2023
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